SeaBASS Submission Checklist: Particulate organic carbon (POC) and particulate nitrogen (PN) data V20190925 Please address the questions below. Rename this file to be specific to your data, e.g., “checklist_poc_MyCruiseName.rtf” and include it with your other submitted documents. SOCCOM ______________________________________________________________________ MR19-04 I7S 49NZ20191229 ______________________________________________________________________ -------------------------------------- - I. Sample measurement and analysis - -------------------------------------- a. Protocols followed and references: https://cce.lternet.edu/data/methods-manual/augmented-cruises/particulate-organic-carbon-nitrogen b. Filter type 1. Filter diameter and nominal pore size: 0.7 µm 2. Make and type (e.g., Whatman GF/F): Whatman GF/F 3. Any pre-treatment applied to the filters (e.g., Combustion temperature, and duration): combustion c. Sampling and processing 1. Method of filtration (e.g., bench top, in line system): vaccum pump 2. Was filtration volume (volfilt) for each replicate (if equal volumes filtered) reported in the data matrix? Mean volume filtered was erported for replicates 3. Were number of replicates per reported sample in the data matrix (i.e., bincount)? yes 4. Were Standard deviation or % cv of replicates reported in the data matrix? standard deviation for replicates only d. Post sampling processing 1. Acidification step: Mode of application (i.e., fumes vs. solution): HCl fumes 2. Drying method and temperature (length of time in oven, desiccator, etc): 48 hours at 60ºC 3. Elemental analysis: e. Elemental analysis 1. Instrument make and model: Costech ECS 4010 CHNSO Analyzer 2. Calibration standard: Acetanilide 3. Reference material used and performance metric results relative to reference: r2=0.996 f. Blank corrections 1. DOC/DON adsorption (“filtrate”) blank correction should be measured and applied (Moran et al. 1999). Were samples blank corrected? wet blank corrected. ------------------ - II. References - ------------------ Moran, S. B., M. A. A. Charette, S. M. Pike, C. A. Wicklund, M. S. Bradley, M. A. A. Charette, S. M. Pike, and C. A. Wicklund. 1999. Differences in seawater particulate organic carbon concentration in samples collected using small- and large-volume methods: the importance ofDOC adsorption to the filter blank S.B. Mar. Chem. 67: 33–42.